SERVICES

Protein Concentration

Method: Ultraviolet Spectrophotometry
Instrument: UV-1800, Shimadzu

Protein Purity and Integrity

Method: Microchip Capillary Gel Electrophoresis
Instrument: Bioanalyzer 2100, Agilent Technologies

This on-chip electrophoresis system is conducive for rapid, precise quality analysis of biopharmaceuticals. Proteins are labeled with a fluorescent dye for enhanced sensitivity. Data acquired includes relative protein size and purity. SDS-denenatured samples are run under reduced and non-reduced conditions. The linear range of the method using the HSP-250 kit is from 10 to 250 kDa and the lower detection limit is ~0.5 ng. The microfluidic design permits analysis of small sample volumes.

Features

Disposable cartridges to eliminate cross-contamination.
Small sample volume, 1 to 5 µl.
Assay 10 samples in 30 minutes.
Sensitivity is 1 pg/µl BSA on Chip
Labelling concentration 1ng- 1µg/µl

Titer Determination and Hydrophobic Footprint Analysis

Method: Reversed Phase – High Pressure Liquid Chromatography
Instrument: Infinity 1260 BioInert, Agilent

Optimization of Protein Purification

Instrument: AKTA Avant, General Electric

Glycan Profile Characterization

Method: MALDI ToF/ToF Mass Spectrometry
Instrument: 4800, Sciex

Glycan Profile Characterization, Protein Identification, and Whole Protein Mass Determination

Method: Biolayer Interferometry and ELISA
Instruments: Octet QKe, ForteBio

Chinese Hamster Ovary (CHO) Host Cell Protein levels are monitored with sensors that are prepared using the Cygnus 3G CHO-specific antibodies and proteins. The advantages of the biolayer interferometry technology is the smaller variability of the reported values.

Protein Identification

Method: Peptide Mass Fingerprinting by MALDI ToF/ToF Mass Spectrometry
Instrument: 4800, Sciex

Optimization of Protein Expression

Instrument: Finesse Bioreactors, Thermo Fisher

Charge Heterogeneity Analysis

Method: Imaged Capillary Isoelectric Focusing
Instrument: iCE3, Protein Simple